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Digest

The Weekly Signal

Published March 6, 2026

Executive Summary

This week's digest highlights: For single-level TLIF procedures, P-15 peptide-enhanced bone graft demonstrates superior composite clinical success compared to autograft, offering a viable alternative in spine surgery. In pediatric H3 G34-mutant diffuse hemispheric gliomas, MGMT protein expression assessment is critical for prognosis, and ensuring adequate radiation field coverage is essential due to predominantly...

Owen briefs you on what matters in this week's digest.

Think chief-resident chalk talk: what matters, what changes practice, and where to spend your reading time.

Endovascular / Vascular

Association of baseline infarct size, reperfusion grade and intracranial hemorrhage in patients undergoing thrombectomy.

Research • Endovascular / Vascular • Journal of neurointerventional surgery • 2026-02-16

In thrombectomy patients, assess baseline ASPECTS to contextualize post-reperfusion hemorrhage risk: low ASPECTS with high eTICI may warrant closer monitoring.

OIn patients with large baseline infarcts (low ASPECTS), achieving high-grade reperfusion paradoxically increases the risk of any intracranial hemorrhage, so monitor these patients closely post-procedure, though this finding did not affect symptomatic hemorrhage rates.

High evidencePractice changing

Study snapshot

Design

Randomized trial (ESCAPE-NA1) retrospective analysis

Population

Patients undergoing thrombectomy in the ESCAPE-NA1 trial

Intervention

Thrombectomy with assessment of reperfusion status (eTICI score)

Comparator

None (single-arm analysis of associations)

Primary outcome

Association of ASPECTS and eTICI on post-treatment intracranial hemorrhage

Why it matters

Previously, we knew that successful reperfusion after thrombectomy improves outcomes but may increase hemorrhage risk. This study adds that the relationship between reperfusion and hemorrhage depends on baseline infarct size: reperfusion reduces hemorrhage risk in patients with small baseline infarcts (high ASPECTS) but increases it in those with large baseline infarcts (low ASPECTS). Clinicians should now consider baseline ischemic extent when weighing reperfusion benefits against hemorrhage risks, though this did not translate to symptomatic hemorrhage differences.

Practice change

Confirms current practice of monitoring for hemorrhage post-thrombectomy, but may support closer imaging follow-up in patients with low ASPECTS and successful reperfusion.

More context

Key details

  • Retrospective analysis of ESCAPE-NA1 randomized trial data.
  • Assessed interaction between baseline ASPECTS and final eTICI score on post-treatment hemorrhage.
  • Any intracranial hemorrhage occurred in 34.2% of patients.
  • Marginal probabilities showed increased hemorrhage risk with low ASPECTS and increasing eTICI scores.
  • Association reversed in patients with small baseline ischemic changes and successful reperfusion.
  • No significant association found with symptomatic intracranial hemorrhage or parenchymal hematoma.

High-yield

In thrombectomy patients, reperfusion reduces hemorrhage risk with small baseline infarcts (high ASPECTS) but increases it with large baseline infarcts (low ASPECTS), though symptomatic hemorrhage rates remain unaffected.

Clinical context

Better reperfusion status results in smaller infarct volumes and better outcomes after thrombectomy. However, if large tissue volumes are already infarcted at baseline, reperfusion might also increase the risk of intracranial hemorrhage.

Limitations

Retrospective analysis of trial data may introduce selection bias despite randomization.Findings are based on imaging-defined hemorrhage rather than clinically symptomatic events.

Methodological critique

Retrospective analysis of randomized trial data limits causal inference for the observed interactions.

Teaching pearl

When reviewing post-thrombectomy imaging, remember that a high eTICI score in a patient with a low ASPECTS may signal higher hemorrhage risk—check the 24-hour CT closely, but don't panic unless it's symptomatic.

Funding and COI

Not stated

Tumor / Skull Base

In pediatric H3 G-mutant DHG, prioritize MGMT protein expression assessment and ensure adequate radiation field coverage, as most recurrences are local.

OIn pediatric H3 G34-mutant DHG, MGMT protein expression (not promoter methylation) is the key prognostic biomarker, and local radiation field coverage is critical as most recurrences are in-field.

Moderate evidencePractice changing

Study snapshot

Design

Retrospective multicenter cohort study

Population

Pediatric patients with newly diagnosed H3 G34-mutant diffuse hemispheric glioma

Intervention

Evaluation of clinical, imaging, molecular features, and treatments (surgery, radiation, temozolomide)

Comparator

None (descriptive and correlative analysis)

Primary outcome

Identification of prognostic factors and patterns of treatment failure

Why it matters

Before this study, pediatric H3 G34-mutant diffuse hemispheric glioma (DHG) had limited clinical data, with unclear prognostic factors and temozolomide response. This study adds the largest pediatric cohort to date, showing that MGMT expression (not promoter methylation) correlates with survival, and most failures occur within the radiation field. Clinicians should now focus on MGMT protein expression and consider radiation field coverage in treatment planning.

Practice change

Could consider incorporating MGMT protein expression testing into the diagnostic workup for pediatric H3 G34-mutant DHG to better inform temozolomide use.

More context

Key details

  • Retrospective multicenter cohort study of pediatric patients with H3 G34-mutant DHG.
  • Assessed clinical, imaging, and molecular features, including MGMT expression and promoter methylation.
  • MGMT expression, not promoter methylation, correlated with survival outcomes.
  • Most treatment failures occurred within the high-dose radiation field.
  • Surgery and temozolomide use were evaluated for associations with outcomes.
  • Included whole genome, exome, transcriptome, and methylation array analyses.
  • Study aimed to identify molecular prognostic markers and patterns of treatment failure.

High-yield

In pediatric H3 G-mutant DHG, MGMT protein expression correlates with survival, not promoter methylation, and most recurrences are within the high-dose radiation field.

Clinical context

Pediatric-type diffuse high-grade gliomas (pHGGs) are molecularly diverse and fatal CNS tumors. Nearly 30% of pHGGs occurring in the cerebral hemispheres of older adolescents and young adults have H3 G34 mutations.

Limitations

Retrospective design with potential selection bias and heterogeneous treatment regimens.Small sample size (n=36) limits statistical power for subgroup analyses.

Methodological critique

Retrospective design and small cohort size limit generalizability and ability to establish causal relationships.

Teaching pearl

For pediatric DHG cases, order MGMT immunohistochemistry alongside methylation testing—the protein expression might tell you more about temozolomide sensitivity than the promoter status alone.

Funding and COI

Not stated

Spine

For single-level TLIF, consider P- peptide-enhanced bone graft as an alternative to autograft given its superior composite clinical success.

OP-15 peptide graft shows promising fusion and clinical outcomes in TLIF, but given the composite endpoint's complexity and the study's industry sponsorship, interpret its superiority over autograft with cautious optimism until replicated in broader practice.

High evidencePractice changing

Study snapshot

Design

Prospective, multicenter, single-blind, randomized controlled trial

Population

Skeletally mature adults (22-80 years) with single-level degenerative disc disease undergoing TLIF

Intervention

P-15 peptide-enhanced bone graft (P-15L)

Comparator

Local autologous bone graft

Primary outcome

Month 24 composite clinical success (CCS)

Why it matters

Local autograft has been the standard for bone graft in TLIF but carries harvest morbidity and variable fusion rates. This study demonstrates that P-15 peptide-enhanced bone graft is superior to autograft in composite clinical success at 24 months, with comparable safety. Surgeons could consider P-15L as an alternative to autograft for single-level TLIF.

Practice change

May support using P-15 peptide-enhanced bone graft as an alternative to autograft in TLIF procedures, given its superior composite success rates.

More context

Key details

  • Prospective, multicenter, single-blind, randomized controlled trial.
  • Patients underwent single-level TLIF (L2-S1) for degenerative disc disease.

High-yield

P- peptide-enhanced bone graft achieved 55.5% composite clinical success versus 37.5% for autograft in TLIF, demonstrating superiority with similar device-related safety.

Clinical context

Local autologous bone graft is the gold standard for bone graft in TLIF but has limitations such as harvest site morbidity and variable fusion rates.

Limitations

Single-blind design may introduce bias in patient-reported outcomes.Higher procedure-related AE rate in the investigational group warrants careful consideration of surgical technique.

Methodological critique

Single-blind design could lead to bias in patient-reported outcomes like ODI, though radiographic assessments were blinded.

Teaching pearl

In single-level TLIF, P-15 graft achieved 25.8% higher fusion rates than autograft—when reviewing postoperative CTs, look for bridging bone at 24 months as this was the key radiographic success criterion.

Funding and COI

Not stated

Functional

Postoperative Weight Gain and Body Composition in DBS Patients: Associations with IPG Recharge Efficiency.

Research • Functional • Stereotactic and functional neurosurgery • 2026-03-04

Consider discussing with male DBS patients that increased body fat may slow IPG recharge times.

OIn male DBS patients, counsel that increased body fat may slow IPG recharge times, though this is based on a small, low-quality study.

Low evidencePractice changing

Study snapshot

Design

Cross-sectional study

Population

Thirty people with DBS

Intervention

Standardized charging protocol to measure IPG recharge rate

Comparator

None (correlational analysis)

Primary outcome

Relationship between biometric parameters and IPG charge rate

Why it matters

Weight gain after DBS implantation has been reported, but its impact on device function was unclear. This study suggests a correlation between increased body fat and slower IPG recharge rates in male DBS patients. Clinicians may consider counseling patients on postoperative weight management to potentially optimize device performance.

Practice change

May support discussing weight management with male DBS patients to potentially optimize device recharge efficiency.

More context

Key details

  • Cross-sectional study of 30 DBS patients
  • Standardized charging protocol used to measure battery charge acquisition rate
  • Biometric data included weight, BMI, and body fat measurements
  • Strong negative correlation found in males (r=-0.6)
  • No significant correlation found in female participants despite higher body fat levels

High-yield

In male DBS patients, higher body fat correlates with slower IPG recharge rates (r=-, p=0.02), suggesting adipose tissue depth may impede charging efficiency.

Clinical context

Weight gain has been reported following DBS implantation, and anecdotal evidence suggests difficulties recharging batteries in patients with larger body mass. The relationship between body mass and charge times had not been systematically investigated.

Limitations

Cross-sectional design limits causal inference between weight gain and DBSSmall sample size and exploratory nature require validation in larger cohorts

Methodological critique

Cross-sectional design prevents determination of causality between DBS and weight gain.

Teaching pearl

When male DBS patients ask about recharge times, remember that body fat correlates with slower charging (r=-0.6, p=0.02), so consider discussing weight management as part of device optimization.

Funding and COI

Not stated

General Neurosurgery

Do not prescribe eslicarbazepine acetate for post-stroke seizure prevention based on current evidence.

OThis negative trial was underpowered, so while eslicarbazepine should not be used for post-stroke seizure prevention, its wide confidence interval means we still don't know if it could help or harm.

High evidencePractice changing

Study snapshot

Design

Phase 2a, double-blind, randomized, placebo-controlled trial

Population

Adults with acute ischemic stroke or intracerebral hemorrhage at high risk of developing unprovoked seizures (SeLECT score ≥5 or CAVE score ≥2)

Intervention

Eslicarbazepine acetate 800 mg/day orally for 30 days

Comparator

Placebo

Primary outcome

Proportion of patients with first unprovoked seizure, death, or discontinuation within 6 months after randomization

Why it matters

Previous preclinical studies suggested eslicarbazepine acetate might have antiepileptogenic properties, but clinical evidence was lacking. This phase 2a trial found no significant difference in preventing post-stroke seizures compared to placebo, though it was underpowered. Clinicians should not yet adopt eslicarbazepine for seizure prevention after stroke, but the study demonstrates feasibility for future adequately powered trials.

Practice change

Confirms current practice of not using eslicarbazepine for post-stroke seizure prevention.

More context

Key details

  • Phase 2a, double-blind, randomized, placebo-controlled trial
  • 125 patients with acute ischemic stroke or intracerebral hemorrhage at high seizure risk

High-yield

Eslicarbazepine acetate showed no significant benefit over placebo for preventing post-stroke seizures at months ( vs , OR , 95% CI -, p=) in this underpowered trial.

Clinical context

Eslicarbazepine acetate is an antiseizure medication that has shown potential antiepileptogenic effects in preclinical models of epilepsy. This study aimed to investigate whether it could prevent or reduce incidence of unprovoked seizures after acute ischemic stroke or intracerebral hemorrhage.

Limitations

Underpowered due to slow recruitment and COVID-19 pandemic impactWide confidence intervals preclude definitive conclusions about treatment effect

Methodological critique

The trial was underpowered due to slow recruitment and COVID- pandemic, producing inconclusive results.

Teaching pearl

When evaluating negative trials, note that this study's wide confidence interval (0.31-1.40) means we cannot rule out a clinically meaningful benefit or harm, highlighting the importance of adequate sample size in prevention trials.

Funding and COI

Funding: BIAL

Basic Science

CXCL1: a novel therapeutic target to increase aneurysm healing after coil embolization.

Research • Basic Science • Frontiers in stroke • 2026-02-10

While not yet clinically applicable, CXCL represents a promising therapeutic target for adjunctive pharmacotherapy to reduce aneurysm recanalization after coiling.

OTargeting CXCL1 to improve aneurysm healing after coiling is a promising preclinical concept, but its clinical relevance remains speculative until validated in human studies.

Low evidencePractice changing

Study snapshot

Design

Randomized, blinded preclinical study in a murine aneurysm coiling model

Population

Female and male C57BL/6 mice (8-12 weeks) with surgically created saccular carotid artery aneurysms

Intervention

Systemic CXCL1 neutralization via intraperitoneal injections of neutralizing antibody

Comparator

Isotype-matched IgG control injections

Primary outcome

Percentage of intrasaccular tissue ingrowth measured histologically 21 days post-coiling

Why it matters

Recanalization after aneurysm coiling remains a significant clinical problem. This preclinical study suggests CXCL1 neutralization may enhance tissue ingrowth and improve aneurysm healing after coil embolization. While not yet ready for clinical application, these findings could support future development of adjunctive pharmacologic therapies to reduce recanalization rates.

Practice change

Confirms current practice; preclinical findings may support future development of adjunctive pharmacologic therapies targeting inflammatory mediators like CXCL1.

More context

Key details

  • Preclinical study using a murine carotid artery aneurysm coiling model
  • CXCL1 expression was significantly higher in aneurysms compared to normal carotid arteries
  • Systemic CXCL1 neutralization via intraperitoneal injections of neutralizing antibody

High-yield

Systemic CXCL neutralization for - days increased tissue ingrowth by approximately - percentage points in a murine aneurysm coiling model, suggesting a potential therapeutic target to improve healing.

Clinical context

Up to 26.3% of coiled aneurysms demonstrate recanalization requiring retreatment. The healing process after coil embolization involves inflammatory cell infiltration and fibrous scar formation, but specific inflammatory pathways are not fully understood.

Limitations

Preclinical mouse model may not fully replicate human aneurysm pathophysiology or healing responsesIntraperitoneal administration route subjects antibodies to first-pass metabolism, potentially affecting bioavailability

Methodological critique

The intraperitoneal administration route subjects antibodies to first-pass metabolism, which may affect therapeutic bioavailability.

Teaching pearl

In this murine model, CXCL1 neutralization for at least 14 days—but not 7 days—significantly increased tissue ingrowth after coiling, suggesting treatment duration is critical when targeting this inflammatory pathway.

Funding and COI

Not stated

Trials to Know

Large Artery Occlusion Treated in Extended Time With Mechanical Thrombectomy Trial

Trial • Trials to Know • ClinicalTrials.gov • 2026-03-06

Why it matters

This recruiting trial may offer eligible patients access to novel interventions.

Why it matters

This recruiting trial may offer eligible patients access to novel interventions.

From the Preprint Wire

Virtual Aerobic Exercise Improves Cognition in Mild TBI: Pilot RCT

Preprint • From the Preprint Wire • medrxiv • 2026-02-23

Why it matters

This preprint presents a promising non-pharmacological approach for cognitive rehabilitation in mild traumatic brain injury (mTBI) patients, addressing a significant clinical gap. For neurosurgeons managing mTBI sequelae, a virtual, symptom-guided exercise protocol could offer a scalable adjunct to standard care. However, as an unreviewed preprint, these findings require cautious interpretation until validated through peer review.

More context

Key details

  • 75% completion rate with 94.2% session adherence and no adverse events, supporting feasibility and safety of virtual delivery
  • Aerobic exercise group showed large effect size improvements in executive function (Trail Making Test B-A) compared to balance controls
  • Secondary benefits included reduced sleep disturbances in the aerobic group, suggesting broader neurocognitive impacts

Why it matters

This preprint, which has NOT undergone peer review, addresses a critical question in preventive neurosurgery: whether GLP-1 receptor agonists (like semaglutide) offer superior cardiovascular protection compared to other antiobesity medications in patients with overweight/obesity and prior cardiovascular disease but no diabetes. Given the high prevalence of obesity and its link to recurrent stroke and other cerebrovascular events, neurosurgeons managing secondary prevention may find these comparative effectiveness data relevant for optimizing medical therapy in at-risk populations.

More context

Key details

  • Study included over 120 million patients from the Truveta EHR database, with 35,240 in the bupropion-naltrexone vs. GLP-1 RA comparison and 27,051 in the phentermine-topiramate vs. GLP-1 RA comparison.
  • Primary endpoint was time to first MACCE (stroke or myocardial infarction) in adults with BMI ≥27 kg/m² and prior cardiovascular disease but no diabetes.
  • After propensity score-overlap weighting, GLP-1 RA showed a 39% lower hazard of MACCE vs. phentermine-topiramate, with an adjusted absolute rate difference of 0.98 events per 1000 person-years.